INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES Repositório Digital da Produção Técnico Científica Biblioteca Terezine Arantes Ferraz

 

Publicações do autor   CARLOS ROBERTO JORGE SOARES    depositadas no Repositório Digital/IPEN - Total : 117 20/04/2024 01:18:00
Seq	Ano	Ipen_doc	Tipo	Circ.	Título
1	2023	29600	Artigo de periódico	I	Molecular cloning and AlphaFold modeling of thyrotropin (ag-TSH) from the Amazonian fish Pirarucu (Arapaima gigas)
2	2023	29601	Artigo de periódico	I	Isolation and characterization of the Arapaima gigas growth hormone (ag-GH) cDNA and three-dimensional modeling of this hormone in comparison with the human hormone (hGH)
3	2022	28435	Artigo de periódico	I	Biophysical properties of electrospun chitosan-grafted poly(lactic acid) nanofibrous scaffolds loaded with chondroitin sulfate and silver nanoparticles
4	2022	28694	Artigo de periódico	I	In vitro cytotoxic data on Se-methylselenocysteine conjugated to dendritic poly(glycerol) against human squamous carcinoma cells
5	2021	27769	Artigo de periódico	I	Periplasmic synthesis and purification of the human prolactin antagonist Δ1‑11‑G129R‑hPRL
6	2021	28344	Artigo de periódico	I	Microfluidic caging lipase in hyperbranched polyglycerol microcapsules for extracorporeal treatment of enzyme pancreatic insufficiency
7	2021	28628	Resumo de eventos científicos	I	SARS-CoV-2 Spike (S) glycoprotein expression, purification and characterization in suspension human embryonic kidney cells 293
8	2020	27524	Iniciação Científica	N	Avaliação do cultivo de células CHO adaptadas a suspensão em diferentes formulações de meios de cultura comerciais livres de soro
9	2019	25193	Artigo de periódico	I	Suppression of prolactin secretion partially explains the antidiabetic effect of bromocriptine in ob/ob mice
10	2019	26443	Artigo de periódico	I	Expression of glycosylated human prolactin in HEK293 cells and related N‑glycan composition analysis
11	2019	26487	Artigo de periódico	I	Poly(3-hydroxi-butyrate-co-3-hydroxy-valerate) (PHB-HV) microparticles loaded with holmium acetylacetonate as potential contrast agents for magnetic resonance images
12	2019	26587	Iniciação Científica	N	Expressão e caracterização da tireotrofina humana (hTSH) recombinante em células CHO cultivadas em suspensão
13	2019	26605	Iniciação Científica	N	Purificação de antagonista de prolactina expresso em bactérias Escherichia coli
14	2019	26714	Livro	N	O IPEN e a saúde
15	2019	26765	Resumo de eventos científicos	I	Expression of the human prolactin antagonist delta 1-11 G129R-PRL in E. coli periplasm
16	2018	24236	Artigo de periódico	I	Determination of recombinant Interferon-α2 in E. coli periplasmic extracts by reversed-phase high-performance liquid chromatography
17	2018	24240	Artigo de periódico	I	Human thyroid-stimulating hormone synthesis in human embryonic kidney cells and related N-glycoprofiling analysis for carbohydrate composition determination
18	2018	24735	Artigo de periódico	I	Brain STAT5 signaling modulates learning and memory formation
19	2018	25046	Artigo de periódico	I	High production and optimization of the method for obtaining pure recombinant human prolactin
20	2018	25059	Artigo de periódico	I	Development and characterization of polymeric microparticle of poly(D,L-lactic acid) loaded with holmium acetylacetonate
21	2018	25186	Iniciação Científica	N	Purificação e caracterização da tireotrofina humana (hTSH) expressa em células de rim de embrião humano (Expi293F™) em suspensão
22	2018	25192	Artigo de periódico	I	Porphyrins are increased in the faeces of patients with prostate cancer
23	2017	22681	Artigo de periódico	I	Distribution of growth hormone-responsive cells in the mouse brain
24	2017	22995	Artigo de periódico	I	N-Glycoprofiling analysis for carbohydrate composition and site-occupancy determination in a poly-glycosylated protein: human thyrotropin of different origins
25	2017	22999	Artigo de periódico	I	Expression, purification and characterization of the authentic form of human growth hormone receptor antagonist G120R-hGH obtained in Escherichia coli periplasmic space
26	2017	24118	Iniciação Científica	N	Expressão da tireotrofina humana (hTSH) em células de rim de embrião humano (Expi293F™) cultivadas em suspensão
27	2017	24264	Resumo de eventos científicos	I	Transient expression of recombinant human prolactin and thyrotropin in human embryonic kidney (Expi293FTM) suspension cells
28	2017	24265	Resumo de eventos científicos	I	Expression of human prolactin in HEK293T using different transfection reagents
29	2017	24397	Resumos em periódicos	I	Carbohydrate composition and site-occupancy determination in pituitary and recombinant preparations of human thyrotropin
30	2017	26981	Resumo de eventos científicos	I	GH controls glycemia and metabolic adaptations to starvation via neurons that express the leptin receptor
31	2016	22997	Artigo de periódico	I	Conceptual proposal of a facility design for accommodating minipigs for pharmaceutical and radiopharmaceutical research
32	2016	23977	Resumo de eventos científicos	N	Role of prolactin in the anti-diabetic effects of bromocriptine: possible action through estrogen receptor alpha in the brain
33	2015	20704	Artigo de periódico	I	N-glycoprofiling analysis in a single glycoprotein model: a comparison between recombinant and pituitary glycosylated human prolactin
34	2015	21362	Texto completo de evento	N	Expressão do antagonista de receptor do hormônio do crescimento humano G120R-hGH no espaço periplásmico de Escherichia coli e sua caracterização físico-química
35	2015	21449	Iniciação Científica	N	Expressão do antagonista de receptor do hormônio do crescimento humano G120R-hGH no espaço periplástico de Escherichia coli e sua caracterização físico-química e biológica
36	2015	22638	Texto completo de evento	I	Conceptual proposal of a facility for handlings of minipigs for pharmaceutical and radiopharmaceutical research
37	2014	20269	Resumos em periódicos	I	Expression and characterization of mouse prolactin (mPRL) in CHO dhfr-cells
38	2014	20291	Resumos em periódicos	I	The influence of Escherichia coli cultivation temperature on interferon alpha 2a expression (IFN-'alfa'2a)
39	2014	20664	Resumo de eventos científicos	I	N-glycoprofiting analysis in a single ghycoprotein model: Glycosylated human prolactin
40	2014	22311	Resumos em periódicos	I	N-glycoprofiling analysis in a simple glycoprotein model: glycosylated human prolactin
41	2014	22335	Resumo de eventos científicos	I	Uncovering the mechanism of action of the anti-diabetic effects of bromocriptine: the role of prolactin
42	2013	18812	Artigo de periódico	I	Isolation of the pituitary gonadotrophic 'alpha'-subunit hormone of the giant amazonian fish: pirarucu (Arapaima gigas)
43	2013	19791	Resumo de eventos científicos	I	A comparison between the oligosaccharide structures of native and recombinant glycosylated human prolactin obtained from CHO cells
44	2013	19796	Texto completo de evento	N	The influence of Escherichia coli cultivation temperature on interferon alpha expression (IFN-'alfa'2a)
45	2013	19797	Resumo de eventos científicos	N	Expression and characterization of mouse prolactin (mPRL) in CHO dhfr-cells
46	2012	18110	Artigo de periódico	I	Expression, purification, and characterization of authentic mouse prolactin obtained in Escherichia coli periplasmic space
47	2012	18119	Resumo de eventos científicos	N	Purification of glycosylated human prolactin from CHO cells adapted to serum-free suspension culture, by reverse=phase HPLC
48	2012	18120	Resumo de eventos científicos	N	Expression, purification, and characterization of a CHO-derived mouse prolactin receptor antagonist S177D-mPRL produced in serum-free medium
49	2012	18184	Artigo de periódico	I	Laboratory production of human prolactin from CHO cells adapted to serum-free suspension culture
50	2012	18704	Resumo de eventos científicos	N	Glicoprotein alpha characterization of the Amazonia fis (Arapaima gigas)
51	2012	21804	Iniciação Científica	N	Síntese e caracterização de prolactina de camundongos em células CHO
52	2011	16538	Artigo de periódico	I	Improved bioprocess with CHO-hTSH cells on higher microcarrier concentration provides higher overall biomass and productivity for rhTSH
53	2011	16585	Resumo de eventos científicos	N	First expression of the antagonist of mouse prolactin (S177D-mPRL) by adherent dhfr-CHO cell using p658 vector
54	2011	17127	Artigo de periódico	I	Novel series of sup(177)Lu-labeled bombesin derivatives with amino acidic for selective targeting of human PC-3 prostate tumor cells
55	2011	17621	Resumo de eventos científicos	I	A practical and fast adaptation of CHO cells expressing human prolactin to grow in suspension and its application to laboratory production
56	2011	17663	Texto completo de evento	N	Influência de meios de cultivo comerciais na produção laboratorial de prolactina humana sintetizada por células CHO cultiadas em suspensão
57	2010	14975	Artigo de periódico	I	Synthesis, purification and characterization of recombinant glycosylated human prolactin (G-hPRL) secreted by cycloheximide-treated CHO cells
58	2010	15555	Artigo de periódico	I	Enhancement of human prolactin synthesis by sodium butyrate addition to serum-free CHO cell culture
59	2010	15608	Resumo de eventos científicos	N	Physico-chemical and biological characterization of glycosylated human prolactin produced at IPEN
60	2010	15978	Resumo de eventos científicos	N	Two-step purification of mouse prolactin expressed in the periplasmic space of Escherichia coli
61	2010	16240	Iniciação Científica	N	Analise da expressao de prolactina humana em celulas CHO tratadas com butirato de sodio
62	2010	16350	Artigo de periódico	I	Lymphocytic prolactin does not contribute to systemic lupus erythematosus hyperprolactinemia
63	2009	13059	Artigo de periódico	I	Evaluation of radioinduced damage and repair capacity in human breast cancer cells, MCF-7 and T-47D
64	2009	14063	Artigo de periódico	I	A molecular mimic of phosphorylated prolactin (S179D PRL) secreted by eukaryotic cells has a conformation with an increased positive surface charge compared to that of unmodified prolactin
65	2009	14128	Texto completo de evento	N	Expressao de prolactina de camundongos no espaco periplastico de Escherichia coli
66	2009	14342	Texto completo de evento	N	Producao em escala laboratorial de prolactina humana em celulas CHO adaptadas para o crescimento em suspensao
67	2008	12980	Artigo de periódico	I	Distinct human prolactin (hPRL) and growth hormone (hGH) behavior under bacteriophage lambda Psub(L) promoter control: temperature plays a major role in protein yields
68	2008	16598	Resumo de eventos científicos	N	Purification and characterization recombinant glycosylated human prolactin synthesized in cho cells
69	2008	16599	Resumo de eventos científicos	N	Influence of activation temperature on the degradation of recombinant proteins in E.coli
70	2008	16600	Resumo de eventos científicos	I	Cycloheximide increases the synthesis of recombinant glycosylated human prolactin (G-hPRL) secreted by CHO cells
71	2008	22244	Iniciação Científica	N	Aplicação dos bioensaios com células Ba/F3-LLP e Nb2 na determinação da potência biológica da prolactina humana
72	2007	11949	Texto completo de evento	I	Evaluation of radioinduced damage an repair capacity in human breast cancer cells, MCF-7 and T-47D
73	2007	12448	Resumo de eventos científicos	I	Bioactive and properly folded prolactin is obtained in E.coli in a pilot bioreactor at 37 graus C by constitutive expression based on the unrepressed ?Psub(L) promoter
74	2007	12449	Resumo de eventos científicos	I	The effect of cycloheximide on the glycosylation of recombinant human prolactin synthesized in cho cells
75	2007	12450	Resumo de eventos científicos	I	Induction of micronucleus in breast cancer cells by sup(60)Co gamma radiation
76	2007	22087	Iniciação Científica	N	Aplicação do bioensaio Ba/F3-LLP na determinação da potência biológica da prolactina humana glicosiladfa (G-hPRL)
77	2006	11377	Artigo de periódico	I	Human macroprolactin displays low biological activity via its homologous receptor in a new sensitive bioassay
78	2006	11417	Artigo de periódico	I	Physico-chemical and biological characterizations of two human prolactin analogs exhibiting controversial bioactivity, synthesized in chinese hamster ovary (CHO) cells
79	2006	11435	Resumo de eventos científicos	N	S179D prolactin (PRL) primarity uses the extrinsic pathway and MAPkinase signaling to induced apoptosis in human endothelial cells
80	2006	11490	Artigo de periódico	I	Point mutation of serine 179 in the human prolactin (PRL) affects recombinant protein expression, folding and secretion, abolishes PRL nickel (II)-binding and increases heparin binding capacities
81	2006	11501	Resumo de eventos científicos	I	Human prolactin (hPRL) and growth hormone (hGH) distinct behavior under bacteriophage lamdda Psub(L) promoter control
82	2006	11502	Resumo de eventos científicos	I	Point mutation of serine 179 in the human prolactin (PRL) affects recombinant protein expression, folding and secretion, abolishes PRL nickel (II)-binding and increases heparin binding capacities
83	2006	11548	Artigo de periódico	I	High-level secretion of growth hormone by retrovirally transduced primary human keratinocytes: prospects for an animal model of cutaneous gene therapy
84	2005	11006	Resumo de eventos científicos	I	Practical two-step purification of recombinant human prolactin and two analogous expressed by chinese hamster ovary (CHO) cells
85	2005	11009	Resumo de eventos científicos	I	High-level secretion of growth hormone by retrovirally transduced primary human keratinocytes: prospects for an animal model of cutaneous gene therapy
86	2005	11011	Resumo de eventos científicos	I	Reversed-phase high performance liquid chromatography (RP-HPLC) analysis and hydrophobicity studies of recombinant human pituitary hormones synthesized in E. coli and CHO cells
87	2004	10053	Resumo de eventos científicos	I	Coculture studies Ba/F3-LP cells and HPRL, G129R-HPRL and S179D-HPRL secreting cho cells
88	2003	09785	Artigo de periódico	I	Periplasmic expression of human growth hormone via plasmid vectors containing the lambdaPsub(L) promoter: use of HPLC for product quantification
89	2002	08537	Resumo de eventos científicos	N	Approaches to maximizing the periplasmic expression in Escherichia coli of the human growth hormone gene from a vector containing the lambda Psub(L)
90	2002	08648	Artigo de periódico	I	High-level expression of human thyroid-stimulating hormone in Chinese hamster ovary cells by co-transfection of dicistronic expression vectors followed by a dual-marker amplification strategy
91	2002	08649	Artigo de periódico	I	Reversed-phase high-performance liquid chromatography method for the determination of prolactin in bacterial extracts and in its purified form
92	2001	07733	Resumo de eventos científicos	N	Rec-hPRL production in E.coli periplasmic space with the use of a temperature sensible promoter
93	2001	07734	Resumo de eventos científicos	N	Physico-chemical study on the First International Standard of Recombinant Human Prolactin, carried out at IPEN-CNEN/SP in collaboration with WHO
94	2000	06777	Tese	N	Sintese de prolactina humana em celulas de ovario de hamster chines (CHO)
95	2000	06990	Resumo de eventos científicos	N	Hollow fiber bioreactors for hormone production in CHO cells
96	2000	06993	Resumo de eventos científicos	N	Utilization of di and tricistronic vectors for the expression of recombinant human thyrotropin (rec -hTSH) in CHO cells
97	2000	06994	Resumo de eventos científicos	I	High level synthesis of recombinant human prolactin in CHO cells and identification of the 22 kDa fragment hPRLsub(11-199)
98	2000	06995	Resumo de eventos científicos	I	A reversed-phase high -performance liquid chromatography (RP-HPLC) method for the determination of human prolactin (hPRL) in osmotic shock fluids and in purified preparations
99	2000	07063	Artigo de periódico	I	High-level synthesis of human prolactin in Chinese hamster ovary cells
100	1999	07347	Artigo de periódico	I	High-yield purification of biosynthetic human growth hormone secreted in Escherichia coli periplasmic space
101	1999	07722	Resumo de eventos científicos	N	Expression of recombinant human thyrotropin (REC-hTSH) in CHO cells using dicistronic mRNA expression vectors with amplifiable markers
102	1999	07723	Resumo de eventos científicos	N	Analysis of recombinant humam prolactin (REC-hPRL) directly in E.coli osmotic shock fluids
103	1998	06130	Artigo de periódico	I	Synthesis and characterization of recombinant, authentic human prolactin secreted into the periplasmic space of Escherichia coli
104	1998	07720	Resumo de eventos científicos	N	Purification of glycosylated and nonglycosylated recombinant human prolactin produced by chinese hamster ovary cells in a hollow fiber bioreactor
105	1997	06347	Resumo de eventos científicos	N	Strategies for achieving high-level expression of recombinant human prolactin (hPRL) in E. coli
106	1996	04693	Resumo de eventos científicos	N	Recombinant human growth hormone. Production and quality control
107	1996	04696	Resumo de eventos científicos	N	Production and characterization of authentic human prolactin (hPRL) secreted in the periplasmic space of Escherichia coli
108	1996	04697	Resumo de eventos científicos	N	A study of the efficiency of different types of bacterial expression vectors based on the 'lambda Psub(L)' and 'TAC' promoters
109	1995	05877	Resumo de eventos científicos	N	Physicochemical immunological and biological characterization of recombinant human growth hormone
110	1995	06046	Dissertação	N	Ensaio imunorradiometrico para a determinacao de proteinas bacterianas contaminantes em lotes de hormomio de crescimento humano recombinante produzido no IPEN-CNEN/SP
111	1995	06086	Resumo de eventos científicos	N	Intra and interlaboratory evaluation of commercial recombinant thyrotropin for the preparation of radioassay reagents
112	1993	02159	Resumo de eventos científicos	I	Radioimmunologic techniques used in quantitative and qualitative analyses of recombinant human growth hormone (rec-hGH) for E. coli contaminant proteins (ECP) and hGH-related forms determination
113	1992	01947	Resumo de eventos científicos	N	Estudo das melhores condicoes na determinacao de proteinas de E.coli em produtos obtidos mediante tecnicas de DNA recombinante
114	1992	01948	Resumo de eventos científicos	N	Immunoradiometric assay (IRMA) for the detection of E.coli proteins in recombinant human growth hormone prepared at IPEN-CNEN/SP
115	1991	03885	Resumo de eventos científicos	N	Isolation and purification of biosynthetic human growth hormone expressed in transformed E.coli cells
116	1991	03907	Texto completo de evento	I	Utilization of DNA-recombinant techniques for the preparation of radioimmunoassay reagents of pituitary hormones
117	1991	03910	Texto completo de evento	I	In-house reagent preparation for the setting up of a magnetic immunoradiometric assay (IRMA) for hTSH